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CD28, PE-Cyanine7, clone: CD28.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0289-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15558376

  • £94.27 / 25 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD28
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P10747
MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
CD28.2
PE-Cyanine7
CD28
Conjugated
CD28
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
940
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