CD28, PE, clone: 37.51, eBioscience™
Syrian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 12-0281-81
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28|
|Golden Syrian Hamster|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.