CD279 (PD-1) Monoclonal Antibody (J43), Super Bright™ 780, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15944194	Affymetrix eBioscience 78-9985-82	100 μg

Description

Description: The IP26 monoclonal antibody reacts with the human alpha beta TCR dimer. The alpha beta TCR is expressed by the majority of peripheral T cells. Applications Reported: This IP26 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IP26 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No. 01-2222-42) as compared to single-color stained cells.

CD279 (PD-1) Monoclonal antibody specifically detects CD279 (PD-1) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD279 (PD-1)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q02242
• Gene Symbols: Pdcd1
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Pdcd1

• Clone: J43
• Applications: Flow Cytometry
• Conjugate: Super Bright 780
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene ID (Entrez): 18566
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody