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CD279 (PD-1), PerCP-eFluor™ 710, clone: eBioJ105 (J105), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-2799-42

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15572696

  • £208.18 / 100 tests


EU Stock 7
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Description

Description

Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of activated human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD279 (PD-1)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q15116
PD1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioJ105 (J105)
PerCP-eFluor 710
PDCD1
Conjugated
PDCD1
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5133
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