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CD279 (PD-1), eVolve 655, clone: eBioJ105 (J105), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 86-2799-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15550837

  • £100.59 / 25 tests


Estimated Shipment date
from Supplier 26-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD279 (PD-1)
eBioJ105 (J105)
eVolve 655
Conjugated
IgG1
RUO
Primary
Human
Flow Cytometry
5μL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Monoclonal
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