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CD279 (PD-1), Biotin, clone: J43, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-9985-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15310150

  • £61.99 / 50ยตg


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis.Applications Tested: The J43 antibody has been tested by flow cytometric analysis of Con A-simulated mouse splenocytes and mouse PD-1 transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD279 (PD-1)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q02242
PD1
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
J43
Biotin
Pdcd1
Conjugated
Pdcd1
IgG
50μg
Store at 2-8°C. Do not freeze.
Primary
18566
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