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CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine5, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 15-9942-82
144.67 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells. Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis. Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.Specifications
CD278 (ICOS) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
Q9WVS0 | |
Icos | |
Affinity chromatography | |
RUO | |
54167 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
7E.17G9 | |
PE-Cyanine5 | |
Icos | |
AILIM, CD278, CVID1, activation-inducible lymphocyte immunomediatory molecule, inducible T-cell costimulator, inducible costimulator | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.