CD278 (ICOS) Mouse anti-Human, Clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9948-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15238267

Quantity Price
1 £ 66.65 / 25µg
EU Stock 1
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Description and Specification

Specification

Clone ISA-3
Regulatory Status RUO
Antigen CD278 (ICOS)
Isotype IgG1
Species Reactivity Human
Format Purified
Applications Flow Cytometry
Applications Functional Assay
Applications Immunohistochemistry (Frozen)
Applications Immunoprecipitation
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Concentration 0.5mg/mL
Quantity 25μg
Storage Requirements Store at 2-8°C.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Conjugate Unlabeled

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27kDa and 29kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.