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CD276 (B7-H3), PE, clone: M3.2D7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5973-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15289209

  • £181.74 / 100µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The M3.2D7 monoclonal antibody was generated against and reacts with mouse B7-H3, a member of the B7 family of the Ig superfamily proteins. B7-H3 is reported to be expressed by antigen presenting cells and developing bone during embryogenesis. The ligand for B7-H3 has not yet been identified and it is thought that B7-H3 down-regulates T cell functions by engaging an unknown counter receptor on T cells. M3.2D7 stains mouse B7-H3 transfected cells and not mouse spleen cells. M3.2D7 also stains human B7-H3 transfected cells.Applications Reported: The M3.2D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M3.2D7 antibody is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of mouse B7-H3 transfected cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of mouse B7-H3 transfected cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD276 (B7-H3)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q8VE98
PSEC0249, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, B7 homolog 3, CD276 antigen, costimulatory molecule
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
M3.2D7
PE
Cd276
Conjugated
Cd276
IgG2a
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
102657
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