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CD276 (B7-H3), Functional Grade, clone: MIH35, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5937-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298697

  • £87.19 / 50µg


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For Research Use Only.


Description

Description

Description: This monoclonal antibody reacts with mouse and human CD276 (also known as B7-H3), a type I transmembrane protein belonging to the B7 family of costimulatory proteins. B7-H3 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of B7-H3 is also secreted by monocytes, dendritic cells, and activated T cells. Although the biological role of this molecule remains controversial, recent studies have identified B7-H3 as the ligand for TREM-like 2 (TLT-2).Applications Reported: This MIH35 antibody has been reported for use in functional assays and flow cytometric analysis.Applications Tested: This MIH35 antibody has been tested for blocking activity in mouse, as well as by flow cytometric analysis on transfected cell lines. This can be used at 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD276 (B7-H3)
MIH35
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
B7RP-2, B7RP2, B7H3
IgG2a
RUO
Primary
Murine
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