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CD276 (B7-H3), PE-Cyanine7, clone: 7-517, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-2769-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15558526

  • £68.96 / 25 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: This 7-517 monoclonal antibody reacts with human CD276, which is also known as B7-H3. This type I transmembrane protein is expressed on immature and mature dendritic cells but not monocytes, granulocytes, or resting lymphocytes. Moreover, CD276 is expressed on non-hematopoietic cells such as osteoblasts, fibroblasts, and epithelial cells. However, CD276 expression can be induced on dendritic cells, monocytes, T, B, and NK cells. This co-stimulatory molecule binds activated T cells, leading to cell proliferation and IFN-gamma production.Applications Reported: This 7-517 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 7-517 antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived dendritic cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD276 (B7-H3)
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q5ZPR3
PSEC0249, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, B7 homolog 3, CD276 antigen, costimulatory molecule
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Flow Cytometry
7-517
PE-Cyanine7
CD276
Conjugated
CD276
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
80381
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