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CD275 (B7-H2), PE, clone: HK5.3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5985-83

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15249229

  • £242.26 / 200µg


Estimated Shipment date
from Supplier 27-07-2018
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Description

Description

Description: The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ∽40kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.Applications Reported: The HK5.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD275 (B7-H2)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9JHJ8
B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653, LICOS, ICOS Ligand
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
HK5.3
PE
Icosl
Conjugated
Icosl
IgG2a, kappa
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
50723
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