CD274 (PD-L1, B7-H1), Functional Grade, clone: MIH5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5982-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in in vitro and in vivo functional assays.Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1, programmed cell death 1 ligand 1, programmed death ligand 1|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.