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CD274 (PD-L1, B7-H1), clone: 1-111A, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9971-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15238297

  • £118.70 / 100µg


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For Research Use Only.


Description

Description

Description: The 1-111A monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary testing suggests that 1-111A and MIH5 (Cat. No. 14-5982) cross-block each other.Applications Reported: The 1-111A antibody has been reported for use in flow cytometric analysis.Applications Tested: The 1-111A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD274 (B7-H1)
1-111A
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B7H1, PD-L1, PDL-1, PDL1
IgG2a
RUO
Primary
Murine
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