missing translation for 'onlineSavingsMsg'
Learn More

CD273 (B7-DC) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: 122, eBioscience™

Rat Monoclonal Antibody

£80.75 - £204.00

Specifications

Antigen CD273 (B7-DC)
Clone 122
Target Species Mouse
Conjugate PerCP-eFluor™ 710
Isotype IgG2a, kappa
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15569476 View Documents Affymetrix eBioscience
46-9972-80
25 μg £80.75
25µg View alternative products
Save Up to 
»
EU Stock 2 Please to purchase this item. Need a web account? Register with us today!
15579476 View Documents Affymetrix eBioscience
46-9972-82
100 μg £204.00
100µg View alternative products
Save Up to 
»
EU Stock 2 Please to purchase this item. Need a web account? Register with us today!
Description

Description

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.
Specifications

Specifications

CD273 (B7-DC)
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
122
PerCP-eFluor™ 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.