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CD273 (B7-DC), Functional Grade, clone: TY25, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5986-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248727

  • £137.33 / 100µg


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ∽25kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis. It has also been reported for inhibition of ligand binding.Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD273 (B7-DC)
TY25
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
B7DC, PD-L2, PDL-2, PDL2
IgG2a
RUO
Primary
Murine
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