CD27 Monoclonal Antibody (O323), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15509106	Affymetrix eBioscience 46-0279-41	25 Tests
13494840	Affymetrix eBioscience 46-0279-42	100 Tests

Description

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD27 Monoclonal antibody specifically detects CD27 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD27
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
• Gene Symbols: CD27
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: CD27

• Clone: O323
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P26842
• Gene ID (Entrez): 939
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.