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CD27 Monoclonal Antibody (O323), APC-eFluor™ 780, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£220.00
Specifications
Antigen | CD27 |
---|---|
Clone | O323 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Description
Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
CD27 Monoclonal antibody specifically detects CD27 in Human samples. It is validated for Flow Cytometry.Specifications
CD27 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P26842 | |
CD27 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
CD27 |
O323 | |
Flow Cytometry | |
APC-eFluor 780 | |
Mouse | |
Human | |
939 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.