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CD27, APC-eFluor 780, clone: O323, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0279-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15559536

  • £181.39 / 100 tests


Estimated Shipment date
from Supplier 24-05-2018
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Description

Description

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis.Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD27
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P26842
CD27 antigen, CD27L receptor, T cell activation antigen CD27, T cell activation antigen S152, T-cell activation antigen CD27, tumor necrosis factor receptor superfamily member 7
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
O323
APC-eFluor 780
CD27
Conjugated
CD27
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
939
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