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CD266 (TWEAK Receptor), clone: ITEM-4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9018-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15228127

  • £68.96 / 25µg


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Description

Description

Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.The ITEM-4 antibody has been show to have blocking function.Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.Applications Tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD266 (TWEAK Receptor)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9NP84, Q9CR75
CD266, FN14, TWEAKR
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Western Blot
ITEM-4
Unconjugated
TNFRSF12A
Purified
TNFRSF12A
IgG2b, kappa
25μg
Store at 2-8°C.
Primary
51330
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