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CD265 (RANK), clone: R12-31, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6612-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15217927

  • £211.96 / 500µg


Estimated Shipment
01-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2.Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis.Applications Tested: The R12-31 antibody has been tested by flow cytometric analysis of mouse RANK transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD265 (RANK)
R12-31
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Receptor Activator of NF-kB
IgG2a
RUO
Primary
Murine
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