CD257 (BAFF, BLyS), FITC, clone: 1D6, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-9017-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN-gamma. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors.Applications Reported: The 1D6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D6 antibody has been pre-titrated and tested by flow cytometric analysis of human BLyS transfected cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD257 (BAFF, BLyS)|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|ApoL related ligand TALL-1, B-cell-activating factor, B-lymphocyte stimulator, BLYS, delta BAFF, Delta4 BAFF, dendritic cell-derived TNF-like molecule, DTL, TALL-1, TALL1, THANK, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNFSF13B, TNFSF20, tumor necrosis factor (ligand) superfamily member 13b, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand 7A, tumor necrosis factor-like protein ZTNF4, ZTNF4|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.