CD254 (RANK Ligand), PE, clone: IK22/5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5952-81
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The IK22/5 monoclonal antibody reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand). TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa. Its extracellular domain is most closely related to TRAIL, FasL and TNF. TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes. TRANCE-deficient mouse lacks osteoclasts. In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells. It was also found to be critical in osteoclast differentiation. The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL.Applications Reported: The IK22/5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IK22/5 antibody has been tested by flow cytometeric analysis of mouse CD254 (TRANCE) transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD254 (RANK Ligand)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|RP11-86N24.2, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNF-related activation-induced cytokine, TNFSF11, TRANCE|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.