CD253 (TRAIL), clone: N2B2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5951-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.Applications Reported: N2B2 can be used in flow cytometric analysis, and blocking of TRAIL in functional assays. (Please use Functional Grade purified N2B2, cat. 16-5951, in functional assays.).Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Apo2L (for Apo-2 Ligand), TNF-Related Apoptosis-Inducing Ligand, Tumor necrosis factor ligand, superfamily member 10, TRAIL protein, CD253 antigen, TN|
|Flow CytometryþFunctional AssayþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.