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CD253 (TRAIL), clone: N2B2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5951-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15207627

  • £187.08 / 500µg


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Description

Description

Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.Applications Reported: N2B2 can be used in flow cytometric analysis, and blocking of TRAIL in functional assays. (Please use Functional Grade purified N2B2, cat. 16-5951, in functional assays.).Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD253 (TRAIL)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P50592
Apo2L (for Apo-2 Ligand), TNF-Related Apoptosis-Inducing Ligand, Tumor necrosis factor ligand, superfamily member 10, TRAIL protein, CD253 antigen, TN
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFunctional AssayþImmunoprecipitation
N2B2
Unconjugated
Tnfsf10
Purified
Tnfsf10
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
22035
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