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CD25 Monoclonal Antibody (PC61.5), Super Bright™ 600, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£126.00 - £308.00

Specifications

Antigen CD25
Clone PC61.5
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15963484
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Affymetrix eBioscience
63-0251-82
100 μg
£308.00
100µg
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15953484
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Invitrogen
63-0251-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

CD25 Monoclonal antibody specifically detects CD25 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD25
0.2 mg/mL
Monoclonal
Liquid
RUO
P01590
IgG1 λ
Affinity chromatography
Antibody
PC61.5
Flow Cytometry
Super Bright 600
Rat
Mouse
16184
Primary
4° C, store in dark, DO NOT FREEZE!
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