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CD25 Monoclonal Antibody (PC61.5), Super Bright™ 600, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
£126.00 - £308.00
Specifications
Antigen | CD25 |
---|---|
Clone | PC61.5 |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15963484
|
Affymetrix eBioscience
63-0251-82 |
100 μg |
£308.00
100µg |
Estimated Shipment: 16-04-2024 Log in to see stock. |
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15953484
|
Invitrogen
63-0251-80 |
25 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
CD25 Monoclonal antibody specifically detects CD25 in Mouse samples. It is validated for Flow Cytometry.Specifications
CD25 | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
P01590 | |
IgG1 λ | |
Affinity chromatography | |
Antibody |
PC61.5 | |
Flow Cytometry | |
Super Bright 600 | |
Rat | |
Mouse | |
16184 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |