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CD25, PE, clone: PC61.5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0251-83

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15218499

  • £174.66 / 200µg


EU Stock 9
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Disclaimers:

For Research Use Only.


Description

Description

Description: The PC61.5 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes and Con A-stimulated splenocyte culture. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
PC61.5
PE
Conjugated
Rat
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-2 Receptor alpha
IgG1
RUO
Primary
Murine
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