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CD25, eFluor 660, clone: eBio7D4 (7D4), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0252-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341860

  • £131.65 / 100µg


Estimated Shipment date
from Supplier 19-11-2018
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Description

Description

Description: The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.Applications Reported: This eBio7D4(7D4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio7D4(7D4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01590
IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
eBio7D4 (7D4)
eFluor 660
Il2ra
Liquid
Il2ra
IgM, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16184
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