CD25 Rat anti-Mouse, eFluor 450, Clone: eBio3C7 (3C7), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0253-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15301710

Quantity Price
1 £ 140.02 / 100µg
EU Stock 2
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Description and Specification

Specification

Conjugate eFluor 450
Isotype IgG2b
Antigen CD25
Regulatory Status RUO
Gene Alias Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra
Applications Flow Cytometry
Host Species Rat
Quantity 100μg
Format Conjugated
Concentration 0.2mg/mL
Monoclonal or Polyclonal Monoclonal
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Species Reactivity Mouse
Clone eBio3C7 (3C7)

The eBio3C7 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.