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CD25, eFluor 450, clone: eBio3C7 (3C7), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0253-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15301710

  • £142.66 / 100µg


EU Stock 2
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBio3C7 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
eBio3C7 (3C7)
eFluor 450
Conjugated
Rat
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra
IgG2b
RUO
Primary
Murine
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