CD25 Rat anti-Mouse, Biotin, Clone: eBio7D4 (7D4), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-0252-81

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15299499

Quantity Price
1 £ 36.08 / 50µg
Estimated Shipment date
from Supplier 04-04-2017
Add to basket


Description and Specification

Specification

Format Conjugated
Quantity 50μg
Clone eBio7D4 (7D4)
Antigen CD25
Isotype IgM
Monoclonal or Polyclonal Monoclonal
Species Reactivity Mouse
Conjugate Biotin
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Applications Flow Cytometry
Concentration 0.5mg/mL
Regulatory Status RUO
Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze.
Gene Alias Interleukin-2 Receptor alpha

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.