missing translation for 'onlineSavingsMsg'
Learn More

CD25 Monoclonal Antibody (eBio7D4 (7D4)), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£91.25 - £303.00

Specifications

Antigen CD25
Clone eBio7D4 (7D4)
Concentration 0.5 mg/mL
Applications Flow Cytometry
Classification Monoclonal
View More Specs

Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15209509
View Documents Promotion Details
Affymetrix eBioscience
13-0252-82
100 μg
£91.25
100µg
View alternative products
Save Up to 
»
Estimated Shipment: 27-03-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15219509
View Documents Promotion Details
Affymetrix eBioscience
13-0252-85
500 μg
£185.00
500µg
View alternative products
Save Up to 
»
Estimated Shipment: 27-03-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15229509
View Documents Promotion Details
Affymetrix eBioscience
13-0252-86
1 mg
£303.00
1mg
View alternative products
Save Up to 
»
Estimated Shipment: 25-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies. Purity is > 95% IgG2a as determined by SDS-gel electrophoresis. Endotoxin: <0.5 EU/μg Applications Tested: This eBio7D4 (7D4) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to μgcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Specifications

Specifications

CD25
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
IL2RA
Primary
4° C, store in dark, DO NOT FREEZE!
IL2RA
eBio7D4 (7D4)
Flow Cytometry
Biotin
Rat
Mouse
P01590
16184
IgM κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.