CD25 Rat anti-Mouse, Biotin, Clone: eBio7D4 (7D4), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-0252-86

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15229509

Quantity Price
1 £ 243.96 / 1mg
Estimated Shipment date
from Supplier 02-05-2017
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Description and Specification

Specification

Quantity 1mg
Antigen CD25
Storage Requirements Store at 2-8°C. Do not freeze.
Applications Flow Cytometry
Isotype IgM
Regulatory Status RUO
Species Reactivity Mouse
Primary or Secondary Primary
Concentration 0.5mg/mL
Gene Alias Interleukin-2 Receptor alpha
Clone eBio7D4 (7D4)
Host Species Rat
Format Conjugated
Conjugate Biotin
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.