CD25 Rat anti-Mouse, Biotin, Clone: eBio7D4 (7D4), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-0252-85

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15219509

Quantity Price
1 £ 146.13 / 500µg
EU Stock 4
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Description and Specification

Specification

Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.5mg/mL
Isotype IgM
Primary or Secondary Primary
Format Conjugated
Clone eBio7D4 (7D4)
Storage Requirements Store at 2-8°C. Do not freeze.
Host Species Rat
Quantity 500μg
Conjugate Biotin
Antigen CD25
Regulatory Status RUO
Gene Alias Interleukin-2 Receptor alpha
Monoclonal or Polyclonal Monoclonal
Applications Flow Cytometry
Species Reactivity Mouse

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.