CD25 Rat anti-Mouse, Alexa Fluor 488, Clone: 7D4, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-0252-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15331990

Quantity Price
1 £ 54.42 / 25µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Gene Alias Interleukin-2 Receptor alpha
Antigen CD25
Species Reactivity Mouse
Isotype IgM
Conjugate Alexa Fluor 488
Regulatory Status RUO
Clone eBio7D4 (7D4)
Applications Flow Cytometry
Monoclonal or Polyclonal Monoclonal
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Quantity 25μg
Primary or Secondary Primary
Concentration 0.5mg/mL

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.