CD25 Rat anti-Mouse, Alexa Fluor 488, Clone: 7D4, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-0252-82

100UG ANTI-MOUSE CD25 ALEXA FLUOR® 488

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15341990

Quantity Price
1 £ 121.67 / 100µg
EU Stock 2
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Description and Specification

Specification

Isotype IgM
Clone eBio7D4 (7D4)
Antigen CD25
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Interleukin-2 Receptor alpha
Host Species Rat
Applications Flow Cytometry
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Concentration 0.5mg/mL
Conjugate Alexa Fluor 488

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.