CD25 Rat anti-Mouse, Alexa Fluor 488, Clone: 7D4, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-0252-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15341990

Quantity Price
1 £ 121.67 / 100µg
EU Stock 1
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Description and Specification

Specification

Isotype IgM
Clone eBio7D4 (7D4)
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Quantity 100μg
Species Reactivity Mouse
Gene Alias Interleukin-2 Receptor alpha
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Conjugate Alexa Fluor 488
Host Species Rat
Regulatory Status RUO
Antigen CD25
Concentration 0.5mg/mL
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Applications Flow Cytometry
Format Conjugated

The eBio7D4 antibody reacts with mouse CD25, the 55kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) followed by subsequent detection with another one. eBio7D4 has been reported to block IL-2 binding but can be overcome by additional IL-2. The monoclonal antibody PC61.5 (cat. 16-0251) is recommended for in vivo studies.