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CD25 Monoclonal Antibody (BC96), Super Bright™ 600, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£245.00
Specifications
Antigen | CD25 |
---|---|
Clone | BC96 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Description
Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.
CD25 Monoclonal antibody specifically detects CD25 in Human samples. It is validated for Flow Cytometry.Specifications
CD25 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P01589 | |
IL2RA | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
IL2RA |
BC96 | |
Flow Cytometry | |
Super Bright 600 | |
Mouse | |
Human | |
3559 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.