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CD25, PE, clone: BC96, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0259-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15516746

  • £143.93 / 100 tests


EU Stock 18
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Disclaimers:

For Research Use Only.


Description

Description

Description: The BC96 monoclonal antibody reacts with human CD25, the 55kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: The BC96 antibody is offered in 2 formats: - μg size: It can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested and can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
BC96
PE
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL; 5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra
IgG1
RUO
Primary
Human
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