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CD25, PerCP-eFluor™ 710, clone: CD25-4E3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0257-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15589096

  • £90.30 / 25 tests


Estimated Shipment date
from Supplier 27-06-2018
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Description

Description

Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on a subet of T cells (many of which are regulatory T cells), activated T and B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes.This monoclonal antibody is cross-reactive to rhesus monkey.Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P01589
IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Non-human Primate
Flow Cytometry
CD25-4E3
PerCP-eFluor 710
IL2RA
Conjugated
IL2RA
IgG2b
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3559
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