CD25 Monoclonal Antibody (CD25-4E3), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15519536	Affymetrix eBioscience 47-0257-42	100 Tests

Description

Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes. This monoclonal antibody is cross-reactive to rhesus monkey. Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.

CD25 Monoclonal antibody specifically detects CD25 in Human, Non-human Primate samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD25
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P01589
• Gene Symbols: IL2RA
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: IL2RA

• Clone: CD25-4E3
• Applications: Flow Cytometry
• Conjugate: APC-eFluor 780
• Host Species: Mouse
• Target Species: Human, Non-human Primate
• Gene ID (Entrez): 3559
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.