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CD25 Mouse anti-Human, Alexa Fluor™ 488, Clone: BC96, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-0259-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15540187

  • £100.00 / 25 tests

Estimated Shipment: 28-09-2021

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Description

Description

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Specifications

Specifications

CD25
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P01589
IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
BC96
Alexa Fluor 488
IL2RA
Liquid
IL2RA
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3559
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For Research Use Only.