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CD25, Alexa Fluor 488, clone: BC96, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-0259-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15550187

  • POA / 100 tests

This item cannot be ordered online because the price is subject to change. Please contact our Product Support team on 01509 555500 for assistance.

Disclaimers:

For Research Use Only.


Description

Description

Description: The BC96 monoclonal antibody reacts with human CD25, the 55kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD25
BC96
Alexa Fluor 488
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-2 Receptor alpha, IL-2Ra, IL2Ra
IgG1
RUO
Primary
Human
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