CD25, eFluor 660, clone: P4A10, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-0250-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This P4A10 monoclonal antibody reacts with canine CD25, the 55kDa interleukin-2 receptor alpha (IL-2Ralpha) chain. As in humans and mice, CD25 in dogs is expressed by early T and B lineage progenitors as well as by activated mature T and B lymphocytes. CD25 associates with the IL-2R beta (CD122) and gamma (CD132) chains to form the high-affinity IL-2 receptor. On its own, CD25 binds IL-2 with low affinity. Along with CD4 and Foxp3, CD25 is a major marker used to define regulatory T cells.The P4A10 antibody has been reported to recognize the same epitope as the anti-human CD25 monoclonal antibody ACT-1, but with greater affinity.Applications Reported: This P4A10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P4A10 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood lymphocytes. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.