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CD247 (CD3 zeta), PE, clone: 6B10.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-2479-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15218739

  • £199.23 / 100µg


EU Stock 5
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Description

Description

Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain. The CD3 complex is composed of three monomeric chains (gamma, delta, and ε) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the η subunit. Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, ε, and zeta chains comprise the TCR/CD3 complex. CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells. Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain.The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain. Therefore, flow cytometric analysis with this antibody requires intracellular staining. In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer.Applications Reported: This 6B10.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 6B10.2 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This atibody can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD247
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P20963
T3Z, CD3H, CD3Q, TCRZ, CD3-ZETA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
6B10.2
PE
CD247
Conjugated
CD247
IgG1
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
919
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