CD24, clone: M1/69, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0242-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: The M1/69 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. M1/69 has also been reported in in vitro functional assays. (Please use Functional Grade purified M1/69, cat. 16-0242, in functional assays.).Applications Tested: The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD24 antigen (small cell lung carcinoma cluster 4 antigen), CD24A, FLJ22950, FLJ43543, MGC75043|
|Flow CytometryþFunctional AssayþImmunohistochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.