missing translation for 'onlineSavingsMsg'
Learn More

CD23, PE-Cyanine7, clone: B3B4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0232-81

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15390900

  • £76.71 / 50µg

EU Stock 2
Add to basket



Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Flow Cytometry
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Low Affinity IgE Receptor, FceRII
IgG2a, kappa

For Research Use Only.