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CD23, PE-Cyanine7, clone: EBVCS2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0238-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15568366

  • £186.79 / 100 tests


EU Stock 2
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Description

Description

Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD23
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P06734
CD23, CD23A, CLEC4J, FCE2, IGEBF, BLAST-2, C-type lectin domain family 4, member J, CD23 antigen, fc-epsilon-RII, immunoglobulin E-binding factor, low affinity immunoglobulin epsilon Fc receptor, lymphocyte IgE receptor
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
EBVCS2
PE-Cyanine7
FCER2
Conjugated
FCER2
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
2208
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