CD23, PE-Cyanine7, clone: EBVCS2, eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 25-0238-41

Artikelnummer. 15558366

Beschreibung

Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Spezifikation

• Antigen: CD23
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P06734
• Gene Alias: CD23, CD23A, CLEC4J, FCE2, IGEBF, BLAST-2, C-type lectin domain family 4, member J, CD23 antigen, fc-epsilon-RII, immunoglobulin E-binding factor, low affinity immunoglobulin epsilon Fc receptor, lymphocyte IgE receptor
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: EBVCS2
• Conjugate: PE-Cyanine7
• Gene: FCER2
• Format: Conjugated
• Gene Symbols: FCER2
• Isotype: IgG1, kappa
• Quantity: 25 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
• Primary or Secondary: Primary
• Gene ID (Entrez): 2208

For Research Use Only.