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CD227 (Mucin 1), eFluor 615, clone: SM3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-9893-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15321130

  • £77.04 / 25µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.Please note this antibody sees a distinct epitope from other Mucin 1 antibodies.Applications Reported: This SM3 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P) and immunocytochemistry (ICC).Applications Tested: This SM3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma tissue and by immunocytochemistry on fixed MCF7 cells at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD227 (Mucin-1)
SM3
eFluor 615
Conjugated
Murine
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Immunohistochemistry (Formalin/Paraffin)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Mucin Glycoprotein, MUC1, EMA (Epithelial Membrane Antigen), Episialin, Tumor-Associated Mucin, PEM (Polymorphic Epithelial Mucin), Breast Cancer-Associated Antigen
IgG1
RUO
Primary
Human
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