CD227 (Mucin 1), Alexa Fluor 488, clone: SM3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-9893-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.Please note this antibody sees a distinct epitope from other Mucin 1 antibodies.Applications Reported: This SM3 antibody has been reported for use in flow cytometric analysis and immunocytochemistry.Applications Tested: This SM3 antibody has been tested by flow cytometry and can be used at less than or equal to 1 μg/mL. This SM3 antibody has also been tested by immunocytochemistry on methanol fixed MCF7 cells and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Mucin Glycoprotein, MUC1, EMA (Epithelial Membrane Antigen), Episialin, Tumor-Associated Mucin, PEM (Polymorphic Epithelial Mucin), Breast Cancer-Associated Antigen|
For Research Use Only.