CD226 (DNAM-1), Functional Grade, clone: 10E5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-2261-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis and functional assays.Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of 3-day CD3/CD28-stimulated mouse splenocytes. This can be used at less than or equal to 0.5μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|DNAM-1, DNAM1, PTA1, TLiSA1|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.