CD223 (Lag-3) Rat anti-Mouse, PE, Clone: eBioC9B7W (C9B7W), eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-2231-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15258729

Quantity Price
1 £ 97.13 / 100µg
EU Stock 2
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Description and Specification

Specification

Clone eBioC9B7W (C9B7W)
Antigen CD223 (Lag-3)
Applications Flow Cytometry
Primary or Secondary Primary
Quantity 100μg
Host Species Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Isotype IgG1
Regulatory Status RUO
Format Conjugated
Monoclonal or Polyclonal Monoclonal
Concentration 0.2mg/mL
Conjugate PE
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Mouse

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54kDa fragment containing all the extracellular domains, and a 16kDa fragment containing the intracellular and transmembrane domains. The 54kDa can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.